The GlycoMark Test Validation

The GlycoMark test is a ten minute, two-step enzymatic kinetic assay that measures 1,5-Anhydroglucitol (1,5-AG) in serum or EDTA plasma.

  • Linearity: Linearity is established using the 50 µg/ml calibration standard. Linearity between 0 and 50 µg/ml is desirable; 50 µg/ml is beyond the upper normal range for 1,5-AG and values less than 10 µg/ml are diagnostic in diabetes. Intermediate points are not required but can be assessed by either diluting the 50 µg/ml standard or multiple patient samples with known 1,5-AG values.

  • Precision: Precision is established within assay and between assay using multiple patient samples. CVs are determined from the means and SDs.
  • Accuracy: Accuracy is established using the high and low control set, and/or by running patient samples and sending the samples to another validated reference lab for correlation comparisons.

For validation or technical assistance, please contact us


The GlycoMark test can be run on any appropriate open channel laboratory analyzer, including the Hitachi 917, Abbott Architect C Series, and Beckman AU series analyzers. Please contact GlycoMark Technical Support to obtain information and instrument settings for these and other laboratory analyzers.